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KMID : 0366919960080010038
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Sungkyun Pharmceutical Journal
1996 Volume.8 No. 1 p.38 ~ p.44
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Degradation kinetics of growth hormone-releasing hexapeptide (GHRP-6) in aqueous solution
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Ha, In Sik
Woo, Byung Ho/Lee, Jung Tae/Kang, Tae Suk/Tak, Kyung Kook/Oh, Choon Kyung/Lee, Kang Choon
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Abstract
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The degradation kinetics of growth hormone-releasing hexapeptide (GHRP-6) as a function of pH and temperature as well as buffer species and ionic strength has been studied by stability-indicating reversed-phase gradient high-performance liquid chromatography. Degradation chromatograms were confirmed in different pH solutions and the observed degradation reaction rates approximately followed first-order kinetics with respect to GHRP-6. The influence of the various buffer species (acetate, citrate, phosphate and borate) was shown to be different and the maximum stability of GHRP-6 was revealed to be in acetate buffer of pH 5.5-6.0. Degradation of GHRP-6 was greater in citrate-containing buffers than in acetate-containing ones. Furthermore, in the citrate-containing buffers, the higher buffer concentration caused greater degradation than the lower ones, but the concentration effect was negligible in acetate-containing buffers. Aqueous solution of GHRP-6 buffered with acetate (0.01 M, pH 5.5) showed a predicted t_90% of 4.73 years at 20℃.
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